Add to cart
For research use only.
| Detection Principle | H2O2/Fe2+ can generate hydroxyl free radical through Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl radical and react with it to generate colored substances 2, 3-dihydroxybenzoic acid. There is a characteristic absorption peak at 510 nm. After adding the substances with scavenging ability, the colored substances are reduced, thus measuring the hydroxyl radical scavenging ability of the sample according to the value of absorption value. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-530 nm, optimum wavelength: 510 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Absolute ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 4.6% | intra-assay CV: 4.1% |
| Sample Volume | 20 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Dog serum | 1 |
| Rat serum | 1 |
| Cynomolgus monkey serum | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
1 Results
Other Formats
1 Results
Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
