Human GLP-1(Glucagon Like Peptide 1) ELISA Kit (E-EL-H6025)
- +1
For research use only. Order now, ship in 3 days
Sensitivity | 0.94 pg/mL |
Detection Range | 1.56-100 pg/mL |
Sample Volume | 100 μL |
Total Assay Time | 3 h 30 min |
Reacitivity | Human |
Specificity | This kit recognizes Human GLP-1 in samples.No significant cross-reactivity or interference between Human GLP-1 and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Sandwich |
Assay Type | Sandwich-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Uniport ID | P01275 |
Research Area | Cardiovascular, Neuroscience |
Other Clones
1 Results
Other Formats
1 Results
- Hypoglycemic effect of C. butyricum-pMTL007-GLP-1 engineered probiotics on type 2 diabetes mellitus
IF:12.2
Journal:Gut Microbes(2025)
DOI:10.1080/19490976.2024.2447814Reactivity:Clostridium butyricum wild-type strain
- Gut Bacteroides ovatus ameliorates renal fibrosis by promoting the production of HDCA through upregulation of Clostridium scindens
IF:7.5
Journal:Cell Reports(2024)
DOI:10.1016/j.celrep.2024.114830Reactivity:Human
- Vitamin D3 improves glucose metabolism and attenuates inflammation in prediabetic human and mice
IF:4.8
Journal:JOURNAL OF NUTRITIONAL BIOCHEMISTRY(2024)
DOI:10.1016/j.jnutbio.2024.109659Reactivity:Human
Sample Type:serum
- Heterologous expression of P9 from Akkermansia muciniphila increases the GLP-1 secretion of intestinal L cells
IF:4.0
Journal:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY(2024)
DOI:10.1007/s11274-024-04012-zReactivity:Human
- Revisiting the markers interleukin-6 and glucagon-like peptide-1 for targeting low-grade inflammation in type 2 diabetes: a meta-analysis and our lab experience
IF:3.1
Journal:ACTA DIABETOLOGICA(2024)
DOI:10.1007/s00592-024-02398-8Reactivity:Human
Sample Type:plasma
- Neuroprotective effect of engineered Clostridium butyricum-pMTL007-GLP-1 on Parkinson's disease mice models via promoting mitophagy
IF:7.400
Journal:Bioengineering & Translational Medicine(2023)
DOI:10.1002/btm2.10505Reactivity:Clostridium butyricum,Mouse
Sample Type:substantia nigra (SN)
- The association between calreticulin and glucagon-like peptide-1 expressions with prognostic factors in high-grade gliomas
IF:1.300
Journal:Journal of Cancer Research and Therapeutics(2023)
DOI:10.4103/jcrt.jcrt_1519_22Reactivity:Human
Sample Type:high?grade gliomas (HGG) tissue,cerebral tissue
- Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study
IF:6.590
Journal:Frontiers in Nutrition(2022)
DOI:10.3389/fnut.2022.973291Reactivity:Human
Sample Type:plasma
- Gut microbiota is associated with differential metabolic characteristics: A study on a defined cohort of Africans and Chinese.
IF:6.055
Journal:Frontiers in Endocrinology(2022)
DOI:10.3389/fendo.2022.942383Reactivity:Human
Sample Type:blood
- Correlation between gut microbiota and glucagon-like peptide-1 in patients with gestational diabetes mellitus
IF:4.560
Journal:World Journal of Diabetes(2022)
DOI:10.4239/wjd.v13.i10.861Reactivity:Human
Sample Type:plasma
Q1:Can the human GLP-1 ELISA kit detect the metabolites of GLP-1 in samples with the addition of DPP inhibitors?
Glucagon-like peptide-1 (GLP-1) is a peptide hormone with a relatively short half-life due to rapid degradation by dipeptidyl peptidase-4 (DPP-4) and neutral endopeptidase (NEP 24.11). Only a small fraction of GLP-1 will fully enter circulation. Our GLP-1 kit is designed to detect intact GLP-1 protein and may not detect its metabolites. Although some customers add sitagliptin (DPP-4 inhibitor) and trifluoroacetic acid to samples to prevent GLP-1 degradation, we do not recommend adding organic agents to samples as they may interfere with ELISA detection.
Q2:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q3:Which variant of the spike protein does this kit detect? Do you have a kit specific to detect the spike protein for the omicron variant?
This kit is designed for the original strain of the new crown virus, and the omicron variant has not been verified. However, we have verified 26 recombinant variants of the SARS-CoV-2 spike protein through the kit. For more information, customers can refer to the kit instructions (https://file.elabscience.com/Manual/covid_19/E-EL-E605 .pdf).
Q4:What is the range of enzyme activity of your IL-2 freeze-dried powder
Currently our freeze-dried powder is a concentration unit with no information on the activity unit for the time being.
Q5:What is the principle of adding stop solution to stop color reaction in ELISA experiment?
On the one hand, the activity of HRP enzyme is destroyed and the catalytic function of HRP enzyme is lost. On the other hand, the final color changes due to a change in pH.
Q6:The absorbance of the cell supernatant is less than that of the sample diluent alone, so how to concentrate the sample?
The amount of medium can be reduced for subsequent drug administration and modeling.
Q7:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q8:May I ask which type of plate to choose when ELISA testing?
According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.
Q9:Is the TGF-β1 ELISA Kit detecting the active form of TGF-β1 or the precursor?
The active TGF-β dimer was detected
Q10:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.
