ATP/ADP Ratio Chemiluminescence Assay kit (E-BC-F004)
For research use only.
| Detection Principle |
Adenosine triphosphate (ATP) is the energy currency of organisms, produced by glycolysis or the tricarboxylic acid cycle and electron transport chain in mitochondria, and is the source of energy in cells. Under normal circumstances, ATP and adenosine diphosphate (ADP) will convert to each other, and the change of the ratio of ATP to ADP is closely related to apoptosis, autophagy, energy metabolism and other pathways, so it is often used as an indicator of cell activity. Under the catalysis of luciferase, ATP react with luciferin and emits fluorescence. Convert ADP to ATP by enzyme, and ATP was detected by the same principle. The ATP/ADP ratio in the cell can be calculated. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Chemiluminescence immunoassay analyzer or multifunctional microplate reader (with the function of detecting luminescence) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 2.9-9.4% | intra-assay CV: 3.5-7.6% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
