UBE2V1 Polyclonal Antibody (E-AB-52433)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T, Mouse spleen, Human spleen Verified Samples in IHC: Human esophagus cancer, Human breast cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Full length fusion protein |
| Abbre | UBE2V1 |
| Synonyms | CIR1, CROC-1, CROC1, CROC1A, TRAF6-regulated IKK activator 1 beta Uev1A, UB2V1, UBE2V, UBE2V 1, UBE2V1, UEV-1, UEV1, Ubiquitin conjugating enzyme E2 variant 1, Ubiquitin-conjugating enzyme E2 variant 1 |
| Swissprot | |
| Calculated MW | 16 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. Excluded from the nucleolus. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family.They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s.The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene.It is thought to be involved in the control of differentiation by altering cell cycle behavior.Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified.Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
Other Clones
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Unconjugated
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