TFCP2L1 Polyclonal Antibody (E-AB-90356)
For research use only.
| Verified Samples |
Verified Samples in WB: various cell lines |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human TFCP2L1 |
| Abbre | TFCP2L1 |
| Synonyms | CRTR1, LBP-9, LBP9, TFCP2L1 |
| Swissprot | |
| Calculated MW | 54 kDa |
| Observed MW |
58 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells (ESCs. With KLF2, acts as the major effector of self-renewal that mediates induction of pluripotency downstream of LIF/STAT3 and Wnt/beta-catenin signaling (By similarity. Required for normal duct development in the salivary gland and kidney (By similarity. Coordinates the development of the kidney collecting ducts intercalated (IC and principal (PC cells, which regulate acid-base and salt-water homeostasis, respectively (By similarity. Regulates the expression of IC genes including subunits B1 and D2 of the V-ATPase complex, OXGR1, CA12, SLC4A1, AQP6 and IC-specific transcription factor FOXI1 (By similarity. Regulates also the expression of JAG1 and subsequent notch signaling in the collecting duct (By similarity. JAG1 initiates notch signaling in PCs but inhibits notch signaling in ICs (By similarity. Acts as a transcriptional suppressor that may suppress UBP1-mediated transcriptional activation (By similarity. Modulates the placental expression of CYP11A1. |
Other Clones
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Other Formats
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Unconjugated
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