MMP-9 Polyclonal Antibody(Capture/Detector) (AN000320P)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse lung, Rat lung Verified Samples in ELISA: Recombinant Mouse MMP-9 protein, Mouse serum, Mouse plasma |
| Dilution | WB 1:500-1:1000, ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Applications | WB, ELISA Capture/Detector |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse MMP-9 protein expressed by Mammalian |
| Abbre | MMP-9 |
| Synonyms | Matrix metaLLoproteinase-9, MMP-9, GeLatinase B (GELB), 92 kDa type IV coLLagenase, Mmp9, CLg4b |
| Swissprot | |
| Calculated MW | 81 kDa |
| Observed MW |
84-92 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions,such as morphogenesis,differentiation,angiogenesis,and tissue remodeling,as well as pathological processes including inflammation,arthritis,cardiovascular diseases,pulmonary diseases,and tumor invasion. MMP9,also known as 92-kDa gelatinase B/type IV collagenase,is secreted from neutrophils,macrophages,and some transformed cells,and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodeling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils and is particularly important in the pathogenesis of inflammatory,infectious,and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines,or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent on the delicate balance between MMPs and their inhibitors,excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence,regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. Besides,the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention. |
Other Clones
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