Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method) (E-BC-K143)
For research use only.
| Detection Principle | Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample. |
| Synonyms | Hcy |
| Sample Type | Serum |
| Detection Method | Colorimetric method |
| Detection Instrument | Biochemistry analyzer (340 nm);Spectrophotometer (340 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Detection Range | 0-50 μmol/L |
| Sample Volume | 39 μL |
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