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For research use only.
| Detection Principle | Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly. |
| Sample Type | Serum,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-600 nm, optimum wavelength: 593 nm） |
| Research Area | Inorganic Ions, Plant Physiology Series, Ferroptosis |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.4 μmol/L |
| Detection Range | 0.4-50 μmol/L |
| Precision | inter-assay CV: 1.5% | intra-assay CV: 1.3% |
| Sample Volume | 55 μL(serum/plasma), 300 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1-2 |
| Rat serum | 1 |
| 10% Mouse liver tissue homogenate | 2-3 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 2-3 |
| 10% Epipremnum aureum leaf tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
1 Results
Other Formats
1 Results
Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
