Recombinant ILK Monoclonal Antibody (AN301171L)
For research use only.
| Verified Samples |
Verified Samples in WB: C6 Verified Samples in IHC: Human kidney |
| Dilution | IHC 1:500-1:2000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ILK protein |
| Abbre | ILK |
| Synonyms | HEL-S, ILK, HEL-S-28, ILK-1, ILK-2, P59, p59ILK, ILK1, ILK2, 59 kDa serine/threonine protein kinase, 59 kDa serine/threonine-protein kinase, DKFZp686F1765, Epididymis secretory protein Li 28, HEL S 28, ILK 1, ILK 2, Integrin linked Kinase, Integrin linked kinase 2, Integrin linked protein kinase, Integrin-linked protein kinase, ILK |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
51 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell junction, focal adhesion, Cell membrane; Peripheral membrane protein; Cytoplasmic side, Cell projection, lamellipodium, Cytoplasm, myofibril, sarcomere. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 12F9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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