Recombinant Cytokeratin 10 Monoclonal Antibody (AN301158L)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse skin Verified Samples in IHC: Human skin |
| Dilution | IHC 1:200-1:1000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Cytokeratin 10 protein |
| Abbre | Cytokeratin 10 |
| Synonyms | KRT, K1C, Cytokeratin, type I cytoskeletal, Keratin type i cytoskeletal, KRT10, BCIE, BIE, CK10, EHK, K10, KPP, CK 10, CK-10, Cytokeratin-10, K1C10, Keratin, Keratin 10, Keratin 10 type I, Keratin type i cytoskeletal 10, Keratin type I cytoskeletal 59 kDa, Keratin10, Keratin-10, type I cytoskeletal 10, BCIE, BIE, CK 10, CK-10, Cytokeratin-10, EHK, K10, K1C10, Keratin 10, Keratin 10 type I, Keratin, Keratin type i cytoskeletal 10, Keratin type I cytoskeletal 59 kDa, Keratin-10, Keratin10, KPP, KRT10, type I cytoskeletal 10, Cytokeratin 10 |
| Swissprot | |
| Calculated MW | 59 kDa |
| Observed MW |
59 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, extracellular space, Cell surface, Localized on the surface of desquamated nasal epithelial cells (PubMed:12427098). Localized on the surface of lung cell lines (PubMed:19627498). |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 11G7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. |
Other Clones
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Unconjugated
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